الفهرس | Only 14 pages are availabe for public view |
Abstract Background: Toll-like receptors (TLRs) are components of the innate immune system and provide the first line of defense against microbial infection through their recognition of microbial products. Type diabetes mellitus (DMT ) and HCV infection are severe health problems worldwide. Aim: To investigate the association between TLR rs (c. C/T) gene polymorphisms and the susceptibility to HCV and DMT . Materials and methods: The study included groups; DMT patients with chronic HCV infection, DMT patients without HCV infection and ages and sex matched fifty healthy controls. DMT was diagnosed according to American Diabetes Association and diagnosis of HCV was performed by detection of HCV antibodies in patient serum using enzyme linked immune assay and confirmed by polymerase chain reaction of HCV. All subjects were suspected to full history and clinical examinations. Liver function tests, fasting and postprandial blood sugar and glycated hemoglobin HbA c were performed for all patients. Blood samples were collected from all subjects to investigate the association between TLR rs (c. C/T) gene polymorphisms and the susceptibility to HCV and DMT , using polymerase chain reaction restriction fragment length polymorphism (PCR- RFLP) and PCR products were analyzed on agarose gel stained with ethidium bromide. Results: No significant difference (p |