الفهرس 
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Abstract
As the most commonly used and predominant sweetener all over the world, sucrose is considered a cornerstone in the human diet. As well known by the majority of people that excessive sucrose consumption is one of the major causes of obesity, diabetes and cardiovascular disease, most people today substitute sucrose intake by several types of artificial sweeteners due to their belief that this is an easy way to control their energy intake and protect them from obesity, diabetes and other metabolic disorders.
The health benefits of artificial sweeteners are in conclusive. There are conflicting researches surrounding the health benefits of artificial sweeteners. Some studies are with the use of artificial sweeteners in moderation and a lot more emerging studies are against the use of artificial sweeteners, claiming that artificial sweeteners can promote weight gain, induce insulin resistance and cause hyperlipidemia.
The aim of this study was to investigate the effect of sucralose commercially known as (Sweetal) and aspartame commercially known as (Sugar-match) as the commonly consumed artificial sweeteners by Egyptians, on weight and diabetic profile after 12 weeks of consumption at different levels, versus the normal sucrose and how these sweeteners affect the fat content and distribution in the body organs of rats.
Sixty healthy adult male albino rats of weighing 150-200g were grouped into 10 groups; each group included 6 rats. The study was implemented over two phases. During the first phase which lasted 12 weeks artificial sweeteners were given to the experimental
animals. After 12 weeks of intake, artificial sweeteners supplementation was omitted for 6 weeks after which the animals were sacrificed and the study progressed.
Phase 1 aimed to investigate the effect of different types of commercial artificial sweeteners (Sweetal and Sugar-Match) on the body weight and biochemical parameters of male rats after 6 weeks then after 12 weeks. The rats were distributed as follow:
group 1 (Control): included 6 male rats that were left as control fed on standard laboratory diet only.
group 2 (Sucrose): included 6 male rats that were fed on standard laboratory diet and sucrose (0.5g/kg body weight).
group 3 (Sweetal low dose): included 6 male rats that were fed on standard laboratory diet and sucralose (Sweetal) 2g/kg body weight (two sachets).
group 4 (Sweetal high dose): included 6 male rats that were fed on standard laboratory diet and sucralose (Sweetal) 4g/kg body weight (four sachets).
group 5 (Sugar-Match low dose): included 6 male rats that were fed on standard laboratory diet and aspartame (Sugar-Match) 0.8g/kg body weight (two sachets).
group 6 (Sugar-Match high dose): included 6 male rats that were fed on standard laboratory diet and aspartame (Sugar-match) 1.6g/kg body weight (four sachets).
The experiment was conducted for 12 weeks; the following measurements were made after 6 weeks and after 12 weeks:
Anthropometry.
Fasting blood glucose.
Glycated hemoglobin (HbAIC %).
Serum Insulin.
HOMAIR.
Complete blood count (CBC) including (Hb, HCT, RBCs, MCV, MCH, MCHC, WBCs, Platelets, Lymphocytes, Neutrophils, Monocytes, Eosinophils, Basophils).
Lipid profile including (LDL, HDL, Triglycerides, Total Cholesterol).
In phase 2 the aim was to determine the possibility of restoration of normal parameters after terminating the intake of artificial sweeteners.
Four groups of rats were fed the following diets for 12 weeks:
• group 7(Sweetal low dose): included 6 male rats were fed on standard laboratory diet and sucralose (Sweetal) 2g/kg body weight (two sachets).
• group 8(Sweetal high dose): included 6 male rats were fed on standard laboratory diet and sucralose (Sweetal) 4g/kg body weight (four sachets).
• group 9(Sugar-Match low dose): included 6 male rats were fed on standard laboratory diet and aspartame (Sugar-Match) 0.8g/kg body weight (Two sachets).
• group 10(Sugar-Match high dose): included 6 male rats were fed on standard laboratory diet and aspartame (Sugar-match) 1.6g/kg body weight (four sachets).
• After 12 weeks the blood was collected from the eyes of the rats and the following parameters were measured:
Fasting blood glucose.
Glycated hemoglobin (HbAIC).
Serum Insulin.
Complete blood count (CBC).
Lipid profile
Body weight was measured weekly.
Histopathalogical Examination was done at the end of the study to demonstrate the histological changes of the liver, kidneys, pancreas and testis tissues.