Search In this Thesis
   Search In this Thesis  
العنوان
Histological Study of an Experimentally Constructed Decellularized Rat Lung as a Model of Whole Organ Three-Dimensional Natural Scaffold
الناشر
Faculty of medicine
المؤلف
Abdelsayed,Marium Romany
هيئة الاعداد
باحث / مريم رومانى عبدالسيد مليكة
مشرف / أ.د./ سوزى صبحى عطاالله
مشرف / أ.د./جيهان خلف مجاهد
مشرف / د/ أسماء عبدالمنعم أبوزيد
تاريخ النشر
2019
عدد الصفحات
126 P.:
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم الأنسجة
تاريخ الإجازة
1/1/2019
مكان الإجازة
جامعة عين شمس - كلية الطب - علم الأنسجة
الفهرس
Only 14 pages are availabe for public view

from 125

from 125

Abstract

Examination of Masson stained sections showed preserved normal lung architecture. Collagen fibers present in the walls of the arteriole, bronchiole and alveolar septa were preserved.
Examination of Orcein stained section revealed the elastic fibers stained reddish brown. They were continuous and distinct in the lamina propria of the bronchioles, inter alveolar septa as well as in the walls of the pulmonary arterioles.
Examination of PAS stained section revealed preserved PAS positive material in the continuous basement membrane of alveolar septa.
In this work, transmission electron microscopy was used to study the capillary alveolar basement membrane, which is a very important feature of a successful decellularized scaffold to assist stem cell movement into the alveolar spaces and help the growth of endothelial cells in decellularized lungs.
Transmission Electron Microscope sections revealed patent alveolar spaces with intact interalveolar septa containing collagen fibrils. Pneumocyte type 1, pneumocyte type 2, and endothelial cells were absent.