الفهرس | Only 14 pages are availabe for public view |
Abstract English Summary Physical studies and photoanalytical techniques for assessment of some important compounds The study presented in this thesis includes development and the combination between some techniques and technologies such as Analytical chemistry, Biochemistry, Photochemistry and Nanotechnology for the determination of some important compounds such as Alpha amylase Enzyme, Cortisol Hormone and Nalbuphine Drug in the human serum. The thesis is divided into four chapters: Chapter One:- Includes a general introduction on lanthanides, optical sensors and other methods used for determination of Alpha amylase Enzyme, Cortisol Hormone and Nalpuphin in the human serum . Chapter Two:- Describes a new nano-optical sensor Tb (III) - carboxyphenyl-18-Crown-6 complex doped in sol-gel matrix for assessment of Alpha amylase activity in human serum and using the alpha amylase as biomarker for the early diagnosis of pancreatitis & parotitis for human. The optimal experimental conditions such as solvent, pH and molar ratio corresponding to maximum luminescence intensity were investigated. Under optimal conditions, the luminescence intensity of Tb3+- carboxyphenyl-18-crown-6 doped in sol gel matrix is inversely proportional to the concentration of 2-CNP in range ( 3.0 X10-4 – 8.0 X 10-9) mol L−1, ( r = 0.999 ). The detection limit(LOD) (S:N=3) and quantitation limit of detection (LOQ) are (1.2 x 10-9 and 3.6 x 10-9) mol L-1 respectively. This method is used for the determination of alpha amylase in human serum. Chapter Three:- Describes novel, simple, sensitive and selective spectrofluorimetric method for the determination of Cortisol hormone serum samples using optical sensor Quinoldine derivative in acetonitrile. Cortisol can remarkably quench the luminescence intensity of Quinoldine derivative in acetonitrile at pH (5.7) and λex= 340 nm. The quenching of luminescence intensity peak of Quinoldine at λem = 617 nm is proportional to the concentration of Cortisol. The dynamic ranges found for the determination of Cortisol concentration are (1.0×10-9– 1.0×10-4) mol L-1. The limit of detection (LOD) and quantification limit of detection (LOQ) are (3.4 x 10-10and 1.0 x 10-9) mol L-1 respectively. Chapter Four:- The efficiency of excited-state interaction between Tb3+and the 4’carboxybenzo- 18crown-6-ether (CCE) has been studied in different solvents and pH. The high luminescence intensity of Tb-complex in DMF at 545 nm was obtained. The photo physical properties of the green emissive Tb3+complex have been elucidated. The Tb- CCE was used as photo probe for the assessment of Nalbuphin HCl in the pharmaceutical formulations and serum samples at pH 6.5 and λex = 285 nm with a linear range 5x10-8 to 1.2x10-6 mol L−1 of Nalbuphin HCl, correlation coefficient of 0.993 and detection limit of 9.4 x10-9 mol L−1 |