الفهرس 
قائمة المحتوياتOnly 14 pages are availabe for public view
 |  | from | 184 |  |  |
| | | from | 184 | | |
Abstract
Non-viral delivery systems for gene therapy have been increasingly
proposed as safer alternatives to viral vectors. They have the potential to be
administered repeatedly with minimal host immune response. They are
targetable, stable in storage and easy to produce in large quantities. Cationic
polymer has been shown as a promising carrier among the non-viral gene
delivery systems. Polycation-DNA complexes generally are more stable
compared with other non-viral gene delivery systems, especially liposomal
or cationic lipid systems. Chitosan (CS) as a polycationic has good
biocompatibility, biodegradability and permeation-enhancing properties.
Because of these properties, it has been widely used in pharmaceutical
research and in industry as a carrier for drug delivery and as biomedical
material. CS has been shown to effectively bind DNA and partially protect
DNA from nuclease degradation.
The work in this thesis is divided into three parts:
Part I: Physicochemical characterization of plasmid DNA loaded chitosansodium deoxycholate nanoparticles
Part II: In-vitro cytotoxicity and transfection efficiency of plasmid DNA
loaded chitosan-sodium deoxycholate nanoparticles
Part III: Physicochemical properties of 5 fluorouracil loaded chitosansodium deoxycholate nanoparticles and in-vivo anti-tumor effect
ii
of both 5 fluorouracil and plasmid DNA loaded chitosan-sodium
deoxycholate nanoparticles