الفهرس 
Nodules collection and preservationOnly 14 pages are availabe for public view
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Abstract
The present study aimed to study the natural bio-diversity and the
genetic convergence of the non rhizobial endophytic bacteria isolated from
root nodules of Mellilotus indicus, from different habitats in Egypt, by
conducting and analyzing some morphological, physiological and genetical
experiments.
The bacteria were isolated and purified from root nodules of Melilotus
indicus plants. The morphological features of the isolates were studied.
Some of the physiological properties such as the effect of salinity, high and
low temperature, pH have been studied. Also, the ability of the different
bacterial isolates to use different carbon sources, to resist some antibiotics,
the heavy metals effect on the growth of these isolates were also studied.
Moreover, to the growth promoting activity of them was studied through
solublization of phosphorus and production of indole acetic acid and
hydrogen cyanide, as well as their Antibiosis activity against some soil born
fungi. All the obtained results were analyzed statistically to determine the
degree of similarity between isolates.
Soil samples were also collected from the different habitats of M. Indicus
collection. Their physical and chemical properties were determined.
Finally, the genetic polymorphism between the non- rhizobial bacterial
isolates were determined by extracting the DNA of different isolates using
different genetic tools like RAPD-PCR and ERIC- PCR to clarify natural
variation that might be present between the isolates.