الفهرس 
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Abstract
This study was carried out to evaluate the effect of garlic with lime juice mouthwash on gingival bleeding, dental plaque and salivary streptococcus mutans counts in a group of Egyptian children, and compare its effect with 0.2 % chlorhexidine mouthwash.
Thirty six healthy children aged from 9-12 years old were selected from outpatient clinic of Pediatric Dentistry and Dental Public Health Department, Faculty of Dentistry, Cairo University after obtaining a written consent and clearance from the Ethical Committee.
The children were randomly divided into two groups, group (A): eighteen children used garlic with lime juice mouthwash, and group (B): eighteen children used 0.2 % chlorhexidine mouthwash, under parent’s supervision twice daily for 14 day.
The gingival bleeding of children was assessed by asking them (Do you have bleeding on brushing?) And they answered with yes or no. Then they were instructed to rinse for 14 days, twice daily, morning after breakfast and night before going to bed, with 10 ml (undiluted) of the assigned mouthwash (Garlic with lime juice mouthwash for group A and Chlorhexidine 0.2% mouthwash for group B) for 30 second, and then expectorate the rinse. A measuring tool was provided to the children to dispense 10 ml of the assigned mouth rinse.
The plaque samples were obtained from buccal surfaces of the upper premolars and molars using disposable sterile cotton swabs. Also two milliliters of unstimulated saliva samples were obtained by spitting into a labeled dry plastic vial. The saliva and plaque samples were transferred in an icebox to the laboratory of the Microbiology Department -Faculty of Medicine-Cairo University-Egypt. The samples inoculated on blood agar plates to determine the count of streptococcus mutans colony.
Again after 14 days, the gingival bleeding of children was assessed by asking them (Do you still notice bleeding when you brush your teeth?) they answered with yes or no and these results were recorded for each child. The saliva and plaque samples were taken and inoculated on blood agar plates to determine the colony count.
The results showed that there was no a statistical significant difference between the two mouthwashes regarding their effects on gingival bleeding. But intragroup showed that there was a statistically significant difference before and after using both mouthwashes.
There was statistical significant difference between the two mouthwashes regarding their effects on streptococcus mutans counts in dental plaque, however there was no statistical significant difference between the two mouthwashes regarding their effects on streptococcus mutans counts in saliva.
The results also showed that the effect of garlic with lime juice mouthwash and 0.2% chlorhexidine mouthwash on gingival bleeding, streptococcus mutans counts in dental plaque and saliva were statistically significant.