الفهرس 
THE GENUS STREPTOCOCCUSOnly 14 pages are availabe for public view
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Abstract
Biofilm formation by Streptococcus agalactiae has a vital role in host-colonization. Pregnant female colonization increases the risk of premature rupture of membrane, stillbirth and low birth weight babies, as well as early onset neonatal disease. Resistance of group B Streptococci (GBS) to antimicrobials is rising, biofilm formation of GBS, not only facilitate its persistence but also add to the resistance to antimicrobials. This study aimed to detect the antimicrobial susceptibility and biofilm forming ability of GBS colonizing the genital tract of high risk and low risk pregnant females. Vaginal swab from 100 pregnant females between 35-37 weeks of pregnancy was cultured on Blood agar and on chromatic STREPTO B media. Disk-diffusion test for isolated GBS was done for penicillin, vancomycin, erythromycin & clindamycin. Biofilm formation of GBS isolates was assessed using static microtiter plate method. For the biofilm forming isolates penicillin, vancomycin Minimal inhibitory concentration (MIC) & Minimum biofilm eradication concentration (MBEC) were detected and compared. Vaginal swabs of examined females revealed 24 GBS (24%) isolates, 18 GBS (75%) in the High risk group (HRG) and 6 (25%) in the low risk group (LRG),The difference between the HRG and LRG in GBS colonization was statistically highly significant. Nine GBS strains (37.5%) were able to form biofilm of them 8 isolates (88.9%)were in HRG while only one strain from the LRG was biofilm forming , the biofilm of GBS (MBEC) is more resistant to penicillin and vancomycin than the planktonic form (MIC) ,this difference was highly significant for penicillin and significant for vancomycin