الفهرس 
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Abstract
Staphylococcus pseudintermedius, is a gram-positive, coagulase-positive bacterium commonly found as part of the normal skin, nares, mouth,pharynx, and anus of healthy dogs, cats, and horse. Certain strains of S. pseudintermedius have become a major threat in veterinary medicine due to their frequent methicillin- and multidrug resistant appearance. Due to its increasing resistance to antibiotics, development of additional therapeutic strategies like vaccination is required to control this pathogen.
The success of staphylococci as pathogens may be attributable, at least in part, to their ability to evade the host’s immune system through a variety of mechanisms including a diverse set of cell surface antigens that able to bind multiple host proteins .
A total of 18 clinical isolates of previously identified S. pseudintermedius that represent nine MLST lineages associated with methicillin resistance were tested.
The majority of S. pseudintermedius strains but not all strains of S. pseudintermedius produce sbi gene . In this study15 out of 18 S. pseudintermedius isolates showed positive sbi production by conventional PCR using specific three different primer set. while all strains of S. pseudintermedius produce coa gene by conventional PCR using specific one primer set.
Amplification of gene encoding sbi, coa genes revealed a size of 1500 bp and their sequencing in the present work exhibited two.