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Abstract The present work was conducted to study the chemical composition of Helianthus Tuberosus and the effect of four levels from (Helianthus Tuberosus) on some nutritional and biological parameters in rats suffering from obesity and chronic liver diseases. Normal male’s albino rats (35) of Sprague Dawley Strain weighted 150 ± 10 g. used in this study. The rats divided into two main groups. The first main group (6 rats) fed on basal diet (as a control negative group). The second main group was fed 6 week on high fat diet HFD to induce obesity in rats, then the rats in the second main group were treated with CCl4 in paraffin oil (50% v/v 2ml/kg) twice a week subcutaneous injection (for two weeks) to induce chronic damage in the liver. Then the rats in this group were divided into 5 subgroups, one of them fed on high fat diet ―control +ve group‖ and the other subgroups fed on high fat diets containing different levels of Helianthus Tuberosus (2.5%, 5%, 7.5% and 10%). The obtained results can be summarized as follows: Chemical composition of Helianthus Tuberosus: The amounts of moisture, protein, ash, crud fiber, oil and Soluble carbohydrates were 10.500, 8.841, 6.22, 3.250, 0.61 and 70.579%, respectively. On the other hand the amounts of total sugars and inulin were 29.250 and 20.490%, respectively Biological Evaluation: Effect of Some Levels of Helianthus tuberosus as Source of Inulin on Nutritional Parameters of Rats Suffering from Obesity and chronic Liver Diseases. The mean value of fed intake in healthy group ―control negative group‖ showed non-significant changes, as compared to obese group which suffer from chronic liver disease ―control positive group‖ and the other treated groups. Non-significant differences in initial weight in all groups. On the other hand, the final weight of the positive control group increased significantly, as compared the final weight of the negative control group. The final weight of all treated groups decreased significantly, as compared to the positive control group. The highest decrease in the final weight recorded for the groups treated with (5%, 7.5% and 10%) Helianthus tuberosus. The mean value of body weight gain % of the positive control group ―obese rats suffering from chronic liver diseases‖ increased significantly, as compared to the negative control group ―healthy rats). All treated groups showed significant decrease in BWG%, as compared to the positive control group. The highest decrease in BWG% recorded for the groups treated with (7.5% and 10%) Helianthus tuberosus. Effect of Some Levels of Helianthus tuberosus as Source of Inulin on Organs Weight/Body Weight% of Rats Suffering from Obesity and chronic Liver Diseases. The mean value of liver, kidney, heart and spleen weight/body weight% in obese group which suffering from chronic liver diseases ―control positive group‖ showed significant increase, as compared to healthy rats ―control negative group‖. Treating obese rats which suffer from chronic liver diseases with (2.5%, 5%, 7.5% and 10%) Helianthus tuberosus improved the mean value of liver, kidney, heart and spleen weight/body weight%. The highest improvement of these organs recorded for the groups treated with the high levels of Helianthus tuberosus. Effect of Some Levels of Helianthus Tuberosus as Source of Inulin on Serum Glucose and Leptin hormone of Rats Suffering from Obesity and chronic Liver Diseases. The mean value of serum glucose and leptin hormone in obese group which suffering from chronic liver diseases ―control positive group‖ increased significantly, as compared to healthy rats ―control negative group‖. Treating obese rats which suffer from chronic liver diseases with (2.5%, 5%, 7.5% and 10%) Helianthus tuberosus decreased the mean value of serum glucose and leptin hormone, as compared to the positive control group. Serum glucose and leptin hormone decreased gradually with increasing the levels of Helianthus tuberosus.The highest decrease of these parameters recorded for the group treated with the high levels of Helianthus tuberosus (10%). Effect of Some Levels of Helianthus Tuberosus as Source of Inulin on Lipid Profile of Rats Suffering from Obesity and Chronic Liver Diseases. Feeding rats on high fat diet increased the mean value of serum cholesterol, triglycerides, low and very low density lipoprotein-cholesterol (LDL-c and VLDL-c), while high density lipoprotein-cholesterol (HDL-c) decreased, as compared to the rats fed on basal diets. Treating obese rats with high fat high fat diet containing deferent levels of Helianthus tuberosus (2.5%, 5%, 7.5% and 10%) led to significant decrease in serum cholesterol, triglycerides, LDL-c and VLDL-c, while HDL-c increased, as compared to non-treated group (obese group). The highest improvement of serum cholesterol, triglycerides, LDL-c, HDL-c and VLDL-c recorded for the groups treated with the highest levels of Helianthus tuberosus (7.5% and 10%). Effect of Some Levels of Helianthus Tuberosus as Source of Inulin on Liver Enzymes of Rats Suffering from Obesity and Chronic Liver Diseases. Feeding rats which were suffer from obesity and chronic liver diseases on high fat diet increased the mean value of serum AST, ALT and ALP, as compared to the rats fed on basal diet.Treating obese rats with high fat high fat diet containing deferent levels of Helianthus tuberosus (2.5%, 5%, 7.5% and 10%) led to significant decrease in serum AST, ALT and ALP enzymes, as compared to non-treated group (obese group). The highest improvement of serum AST, ALT and ALP recorded for the group treated with 7.5% Helianthus tuberosus , followed by the group treated with 10% Helianthus tuberosus, respectively. Effect of Some Levels of Helianthus Tuberosus as Source of Inulin on Kidney Functions of Rats Suffering from Obesity and chronic Liver Diseases. Injected rats which were suffer from obesity with ccl4 to induce chronic liver diseases increased the mean value of serum uric acid, urea nitrogen and creatinine, as compared to the healthy rats fed on basal diet. Treating obese rats which were suffering from chronic liver diseases with high fat high fat diet containing deferent levels of Helianthus tuberosus (2.5%, 5%, 7.5% and 10%) led to significant decrease in serum uric acid, urea nitrogen and creatinine, as compared to non-treated group (obese group). The highest improvement of serum uric acid, urea nitrogen and creatinine recorded for the group treated with 7.5% Helianthus tuberosus , followed by the group treated with 10% Helianthus tuberosus, respectively. |