الفهرس 
(Anatomy and physiology of the cornea)Only 14 pages are availabe for public view
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Abstract
Mitomycin-C (MMC), is commonly used adjunctively to trabeculectomy to prevent scarring of the filtering bleb, and improve the success rate of the surgery but MMC has a cytotoxic effect that is independent of cell cycle. This study was to evaluate the effect of MMC on corneal endothelial density and morphology in glaucoma patients who underwent trabeculectomy.
Topcon sp-2000 a non-contact specular microscope was used for analysis of the corneal endothelial cell layer by measurement of mean cell density (MCD) and coefficient of variation (CV) in the cell. These parameters provide an index of the functional status of corneal endothelial layer. This study was performed on 50 eyes and parameters were compared before and 1 month, 3 and 6 months after surgery.
CECD was significantly lower after 1 month of surgery (P<0.001) as the mean CECD decreased from 2808 ± 553 ± 553cells/mm2 to 2487 ± 474 cells/mm2 .This decrease continued from 1 to 6 months but was not significant as the mean CECD at 3 months was 2484 ± 463 cells/ mm2 and 2482 ± 477 cells/mm2 at 6 months (P=0.114). The mean variation of cell size [coefficient of variation (CV)] showed tendency to increase, as it was 48.07 ± 11 before surgery, increasing to 51.43 ± 12 after 1 month, 52.37 ± 12.8 after 3 months and 53.1 ± 12.3 after 6 months. This variation was significant (P<0.001) but not significant from 1 to 6 months (p=0.256)
This showed that although MMC application in trabeculectomy caused significant but also not clinically remarkable loss of endothelial cells that occurred only intraoperatively or at early post-operative period, prolonged toxic effect of MMC and progressive cell loss was not a major concern. During the follow up period, active endothelial adaptations were taking place.