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Abstract A study was conducted on 200 samples of one day old chicks obtained from chicken hatchlings to isolate and identify bacteria associated with yolk sac infection (Omphalitis) and to determine antimicrobial sensitivity pattern of the predominant bacterial pathogens. A total of 160 bacterial isolates were isolated and identified using biochemical tests and molecular confirmation. The bacterial strains tested for their susceptibility to 10 antimicrobial agents. The highest recovery rate was for Escherichia coli 120 (60%) followed by Pseudomonas aeruginosa 25 (12.5%). Staphylococcus aureus and Salmonella were 10 (5%) & 5 (2.5%), respectively. The antimicrobial sensitivity patterns were detected for all bacterial genera; the highest resistance patterns were exhibited by P. aeruginosa followed by Salmonella spp. then E. coli and finally S. aureus. The existence of multi-drug resistance bacteria isolates associated with yolk sac infection suggests that more emphasis be given towards preventing omphalitis in chicks through improvements of sanitary measures than to consider control options through the use of antimicrobials. |