الفهرس | Only 14 pages are availabe for public view |
Abstract Escherichia coli, a member of the family Enterobacteriaceae, is Gram negative facultative anaerobe bacteria. Avian pathogenic Escherichia coli is a subgroup of extra-intestinal pathogenic E. coli, which are responsible for high economic losses in the chicken industry. APEC belongs mostly to the serotypes O1, O2, O78. Bacteriological examination for 200 chicken’s samples revealed that 73 (36.5%) E. coli isolates were identified. The conventionally identified E. coli strains in this work were serotyped using commercial antisera. Twenty six O serogroups were identified among the 73 avian pathogenic E. coli isolates which isolated from 200 chicken samples. Among the isolates that could be typed, the most prevalent serogroups were O78 then O2 and O1. Phenotypically, the recovered E.coli strains were tested for their antimicrobial resistance against 15 antimicrobial agents. Genotypically, E.coli isolates were subjected for multiplex PCR for detection of antibiotic resistance genes including, blaTEM, blaSHV, aadA, tetA and sulI. The recovery rate of this antibiotic resistance genes were 78%, 23%, 54%, 60% and 87% respectively. The sul1gene was the most prevalence gene (87%). In contrast, blaSHV had the lowest prevalence (23%). In Conclusion, in this study, the antimicrobial susceptibility pattern showed high multidrug resistance (MRD) so it is very important to control APEC because it represents a grave danger to Poultry and is a potential source of transferring multi-drug resistance genes to human specific E. coli. The fact that this pathogen is naturally present in daily consumed food should be considered as a serious public health and food biosafety. |