الفهرس | Only 14 pages are availabe for public view |
Abstract Poultry is one of the most important reservoirs of Salmonellae that can be transmitted to humans through the food chain causing high risk of bacterial food poisoning. This study aimed to isolate Salmonella from different poultry species with molecular detection of most common virulence genes from the isolated Salmonellae then sequencing of one of these genes (bcfC gene) and determine the pathogenicity of Salmonella Typhimurium isolated from different species (chickens, ducks and quails) contain most common virulence genes which inoculated orally and intraperitonially in 3 days-old chicks. A total of 265 samples (150 chickens, 60 ducks, 30 quails and 25 turkeys) were examined from Dakahlia, Kafrelsheik, Damietta, Elsharkia and Elgharbia Governorates. Examined birds were subjected to either clinical and/or post-mortem examination for isolation and identification of paratyphoid Salmonellae from internal organs including liver, lung, spleen, caecum and unabsorbed yolk sac. The samples were subjected to bacteriological, biochemical and serological identification. Twenty eight birds (10.6%) out of 265 birds were found positive while 237 (89.4%) birds were negative for Salmonella isolation. The number and percentage of positive chickens, ducks, quails and turkeys were 16 (10.7%), 7 (11.7%), 3 (10%) and 2 (8%) respectively. In our study, S. Typhimurium, S. Enteritidis, S. Kentucky, S. Paratyphi A, S. Molade, S. Heidelberg, S. Infantis and S. Apeyeme were isolated from chickens. While S. Enteritidis, S. Typhimurium, S. Paratyphi A, S. Kentucky, S. Inganda and S. Bargny were isolated from ducks but S. Virchow, S. Tamale and S. Typhimurium were isolated from Quails and S. Wingrove and S. Kentucky were isolated from Turkeys. |