الفهرس | Only 14 pages are availabe for public view |
Abstract Electromagnetic fields have the ability to control cellular pathways, change cell morphology and its different electric properties. Thus, they can be used as anti-cancer therapy by using field intensity and frequency that is able to induce cancer cell apoptosis. Conventional methods of applying electric fields is by placing, the target organ/ tissue between two oppositely charged electrodes. Magnetoelectric nanoparticles (MENs) are considered a new method of applying electric fields. They are a source of both uniform and non-uniform electric fields. Only few studies address using MENs in biological application. In these studies MENs were loaded with anti-cancer or anti-HIV drug. Only one paper used electric field generated by MENs to influence biological activity without loading a drug. In the present study, we are aiming to examine if the electric field generated by MENs as a source of electric field (of uniform and non-uniform field) can affect histopathology and tissue capacitance of hepatocellular carcinoma. To do so we need to know the effects of applied MFs and MENs each alone, in order to study the effect when both applied together. We used two MFs of different intensities and frequencies, so that we can get electric fields of different intensities and frequencies. Histopathology confirmed the dysplastic changes of hepatocellular carcinoma. Each of the MFs and MENs affected histopathology of hepatocellular carcinoma in a different manner. While applying both together lead to unexpected changes, due to the contribution of the induced EFs. Also the alternating and static MFs didn’t lead to the same changes, and so did the induced alternating and static EFs. The capacitance of the control group (HCC) was higher than that of healthy liver, as many studies showed. Intervention with MFs with and without MENs affected these values. Results are in line with the histopathological findings except for group F where histopathology showed severe dysplasia and signs of malignancy, while capacitance values were lower than other groups of mild-moderate dysplasia, this might be attributed to degenerative features found in this group. |