الفهرس 
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Abstract
The Objective of this study was to measure the protein profiles of seminal plasma in Egyptian buffalo bulls semen and to examine their correlation with semen characteristics of buffalo bulls in Mahalet Mousa. Semen of 20 buffalo bulls (divided into high and low fertile bull, 10 for each group, according to records of insemination) were collected twice weekly for 12 weeks by artificial vagina. Semen characteristics of ejaculate volume, motility, abnormalities, viability and sperm cell concentrations were recorded. Seminal plasma was separated by centrifugation, treated with cold ethanol and then underwent SDS-polyacrylamide gel electro phoresis (PAGE).
The attained results can be summarized in the following:
1-The differences between high and low fertile buffalo bulls and between summer and winter in ejaculate volume and sperm cell concentration were not significant will the percentage of mass motility and viability were significantly (P>0.05) higher in high fertility bulls compared to that in low fertile bulls but the differences between summer and winter season were not significant. Moreover, the percentage of sperm abnormalities was significantly (P>0.05) lower in the high fertile bull compared to low fertile bull semen.
2-Total protein concentration in winter season was 6.55±0.40 g/dl while, it was 7.21±0.19 g/dl in summer season. Estimated total protein concentrations were not significantly difference between two seasons (winter vs. summer season). Total protein concentration was 7.40±0.31 and 6.39±0.30 g/dl for high and low fertile buffalo bulls, respectively the differences were significant (P>0.05).
3-Different seasons (winter vs. summer) of the year may influence the presence of a variety of proteins in seminal plasma. There was a direct relationship of the season upon seminal plasma proteins. The presence of the proteins of 55 KDa and HBPs suggests an increase of the semen quality of high fertility bulls during both winter and summer seasons
In conclusion, there were significant differences in seminal plasma profiles between buffaloes with high versus low fertility and semen quality. Furthermore, osteopontin (55 KDa) and HBPs (17KDa) were potential seminal plasma markers of high and low fertility bulls with high and low semen quality.
Key Words: Buffalo bull semen, Protein, Semen characteristics, Seminal Plasma, SDS-PAGE.