الفهرس 
Dendritic Cell PhysiologyOnly 14 pages are availabe for public view
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Abstract
Background: Dendritic cells are professional APCs able to modulate adaptive responses through recognition of pathogens, directly or indirectly, by sensing perturbations in the microenvironment such as infectious agents, cell damage, or inflammation. Aim of the work: to investigate which method of monocyte isolation, adherence method or magnetic acti¬vated cell sorting is the best as regards generation of DCs. Subjects and Methods: Samples in the present study were collected from healthy blood donors from the central blood bank at Ain Shams University Hospitals. All the donors aged between twenty and forty years, of both sexes (18 males and 2 females). They were found healthy in an orienting physical examination. All blood products were negative for common blood-borne pathogens (HBsAg, HIV Ab, HCV Ab), as detected by standard assays. Work area was at Clinical Pathology Department, Ain Shams University Hospital. Results: The present study revealed a statistically significant difference between Method 1 and Method 2 as regards the rate of decrease in CD14+ cell population % from preculture to day 2, being higher in method 1. There was also a statistically significant difference between Method 1 and Method 2 as regards the rate of increase in CD14- cell population %, from preculture to day 2 with method 1 having higher rate of increase, whereas method 2 has shown a significantly higher rate of increase from day 2 to day 4 of culture. Conclusion: This study has shown that adherence method gave higher results of cell count and viability along the whole culture duration in comparison to MACS method. Despite of that, MACS method demonstrated higher results than plastic adherence in generating monocyte derived DCs, as it has shown significantly higher % of CD14- CD11c+ HLA-DR+ DCs population inspite of having a relatively lower mean % of CD14- population. A finding which could suggest that cells separated by MACS were much more capable of being readily transformed into DC than those separated by adherence method. Recommendations: Experiments on induction of further maturation of DCs generated from peripheral blood monocytes using TNFα, LPS, or CD40L are warranted for further use of mature DCs in preparation of DC vaccines for cancer therapy and for treatment of some infectious diseases.