الفهرس | Only 14 pages are availabe for public view |
Abstract Estimation of the postmortem interval (PMI) is a challenging task and represents one of the biggest concerns that may face the forensic pathologists. The present study was carried out to estimate PMI through biochemical changes in some body fluids specially in cases of traumatic death and correlate the effect of cause of death with the PMI. In this study thirty adult male White New Zealand rabbits were weighed and randomly distributed into two main equal groups each containing fifteen rabbits, the first group served as control (cervical dislocation), while the other group killed by weight DROP method. Each group was classified into three subgroups within three postmortem intervals ( at time of death, 6 and 12 hrs postmortem). The rabbits of control and traumatized groups were sedated by intramuscular injection of a mixture of ketamine hydrochloride (35 mg / kg of body weight) and xylazine (5 mg / kg of body weight) anesthetic agents to minimize animal suffering. The rabbits of control groups were euthanized by cervical dislocation, by applying pressure to the neck and dislocating the spinal column from the skull or brain so as to provide the animal with a fast and painless death. While the rabbits in traumatized groups were killed by a trauma on the frontal border of the head that lead to death, using designated specific device to fulfill this aim. All rabbits were kept at room temperature approximately 24oC and 42%. humidity. The experimental procedures carried out according to the general guidelines of the National Institutes of Health (NIH) for the Care and Use of Laboratory Animals in Scientific Investigations and approved by Ethics of Animal Use in Research Committee of Zagazig University. |