الفهرس 
Material and MethodsOnly 14 pages are availabe for public view
 |  | from | 228 |  |  |
| | | from | 228 | | |
Abstract
Epidemiological data have shown an association between the increasing of bisphenol A (BPA) level in the environment and the incidence of hepatotoxicity and renal toxicity. The present study aimed to estimate the protective effect of quercetin on oxidative stress, markers of inflammation, apoptotic and antiapoptotic markers in the hepatic and renal tissues of the bisphenol A treated rats. The current study was conducted on 60 adult male Wistar rats (3 months old) divided into six groups n=15 Gp. (1): normal control rats (con.), Gp. (2): rats were inoculated orally with 1ml olive oil daily for 2 months (olive), Gp. (3) rats were administrated orally with 1ml corn oil daily for 2 months (corn), Gp. (4): rats were administrated orally with quercetin (50mg/kg b.wt.) daily for 2 months (QU). Gp. (5): rats were inoculated orally with bisphenol A (50mg/kg b.wt.) daily for 2 months (BPA) and Gp. (6): rats were inoculated orally with BPA (50mg/kg b.wt.) after 1hour from BPA administration, these animals were treated with QU (50mg/kg b.wt.) daily for 2 months (BPA+QU). At the end of the experimental period, rats were sacrificed then the serum, liver and kidney samples were achieved for different biochemical analyses. The biochemical analyses comprised determination of serum total protein, albumin, total lipids, alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea and creatinine. Also, tissue malondialdehyde (MDA), glutathione-S-transferase (GST), glutathione peroxidase (GPx), interleukin 1 Beta (IL 1β), interleukin 6 (IL 6), caspase 3 (CASP3) and B-cell lymphoma-2 (Bcl-2) were detected.
In comparison with normal control group, oral administration of BPA caused oxidative stress leading to significant decrement in serum total protein and albumin while total lipids recorded significant elevation. Moreover, liver function tests showed significant up-regulation together with urea and creatinine. Moreover, a significant elevation in the activity of malondialdehyde (MDA), glutathione-S-transferase (GST), hepatic IL-6, renal IL-1β as well as, caspase 3 associated with significant reduction observed in Glutathione peroxidase (Gpx) activity and B-cell lymphoma 2 (B-cl2) in both liver and kidney tissues.
In contrast, co-administration of quercetin with BPA showed significant alleviation in serum parameters (total protein, albumin,total lipids, ALT, AST, urea and creatinine). Also, improved the oxidative stress induced by BPA via decreasing MDA levels and enhancing the activity of enzymatic antioxidants. Moreover, treatment of quercetin with BPA resulted in an improvement of IL-6, IL 1β and caspase-3 levels associated with a significant increase in Bcl2 expression in both of liver and kidney tissues.
Conclusion: These data suggest that quercetin protects rat liver and kidney from BPA-induced oxidative stress, probably via its antioxidant properties, anti-inflammatory and antiapoptotic effects. So, quercetin is considered to be a promising pharmacological agent for avoiding the potential hepatotoxicity and renal toxicity due to occupational or environmental exposure to bisphenol A.
Keywords: Bisphenol A, Quercetin, Hepatotoxicity, Renal toxicity, Rats