الفهرس 
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Abstract
Tomato( Lycopersicon esculentum Mill. ) is one of the most popular and important commercial vegetable crops grown throughout the world. There are several diseases on tomato caused by fungi , bacteria, viruses , nematodes. In this context, the aim of the present thesis was to evaluation of some bio-agents and biofertilizers for controlling of wilt and root rot diseases of tomato plants as well as whether have the potential of increasing growth performance and productivity .
The obtained results could be summarized as follows:
1- Twenty fungal isolates were isolated from the infected roots of tomato plants. These fungi were identified as, six isolates of Fusarium oxysporum f. sp.lycopersici , five isolates of Verticillium dahliae, three isolates of Rhizoctonia solani and two isolates of each Sclerotium rolfsii, Macrophomina phaseolina and F. solani.
2- Forty isolates of Azotobacter and Azospirillum were isolated from the rhizosphere and rhizoplane of tomato plants . After purification, the isolates were tested for their efficiency for nitrogen- fixation using the ambient assay of nitrogenase activity and the most efficient five isolates of either Azotobacter SP (AZ9, AZ10, AZ13, AZ14,AZ17)or Azospirillum SP (AS1,AS5, AS7,AS11,AS14). were found to be the most efficient ones.
Bacillus subtilis , Paenibacillus polymyxa, Bacillus megaterium and Pseudomonas fluorescence were obtained from Cairo MIRCEN Microbial Resources Center, Ain -Shams University.
Two fungal isolates which have antagonistic activities and used as bio-agents fungi were obtained from rhizosphere of tomato plants. These isolates were identified as Trichoderma harzianum according to their cultural and microscopically characters.
3- Pathogenicity tests indicated that all tested fungal isolates were able to infect tomato plants (super jackl) and caused wilt and root rot diseases. Verticillium dahlia isolate No.(1) causing Verticillium wilt gave the highest percentage of disease severity, followed by Fusarium oxysporum f. sp. lycopersici isolate No.(9) which causing Fusarium wilt. While, Fusarium solani isolate No.(6) gave the highest percentage of root rot severity followed by Sclerotium rolfsii isolate No.(19) . were selected for further studies under lab .and greenhouse conditions .
4- The results of antagonistic activity of the bioagent Fusarium oxysporum f. sp. lycopersici ,Verticillium dahliae , F. solani and Sclerotium rolfsii in vitro exhibited higher variation in their abilities to reducing the growth rate of the pathogen and increasing the percentage of pathogen inhibition as compared with the control treatment, clear zones around bioagent growth were showed .
5- Trichoderma harzianum (T1)and T. harzianum(T2) which isolated from tomato plant rizosphere and rhizoplane were tested against F. oxysporum f.sp. lycopersici, V. dahliae , F. solani and S. rolfsii. Trichoderma harzianum. (T1)was the most effective on the pathogen growth followed by (T2).At the same time (T2)gave the lowest effect on the mycelial growth of S. rolfsii.
6- Under greenhouse conditions, the inoculated tomato seedling with a mixture bioagents. appeared lower percentage of infected plants than those inoculated with them individually. Growth characteristics , macro-nutrients content and photosynthetic pigments of tomato were significantly increased in the inoculated treatments with bioagent mixture as compared with the bioagent treatments individually.