الفهرس | Only 14 pages are availabe for public view |
Abstract Virulence of L. monocytogenes is controlled by many factors, yet inlA, inlB and hlyA genes remain the major determinants of pathogenicity. These virulence genes are responsible for intestinal adhesion, invasion and systemic spread of the pathogen within host. So, the four detected isolates of L. monocytogenes were subjected to molecular identification by PCR DNA extraction. The four isolates were harbored inlA gene (100 %), while one isolates was negative for inlB and another isolates was negative for hlyA. The results of antibiotic sensitivity test showed that all L. monocytogenes isolates (100 %) were resistant to Ampicillin, Cephalothin and Sulfamethoxazole-Trimethoprim and 75% of them showed resistance for Erythromycin and Tetracycline. However, none of them (0%) resisted Gentamicin, Kanamycin and Chloramphenicol. The control of L. monocytogenes in food needs commitments at different levels of milk production. Milking hygiene habits should be guided and preparation areas should be cleaned and sanitized before and after use. |