الفهرس | Only 14 pages are availabe for public view |
Abstract The present study is concern of developing nanohydroxyapatite (n- HA) powder from biogenic sources such as fish bone skeletons and preparation of n- HA scaffolds coated with PCL to be used as degradable bone substitution. Prepare highly porous scaffolds having a porosity of 85 % from hydroxyapatite powder prepared from biogenic resources such as fish bone skeletons by using cheap and simple eco-friendly method. Million tons of fish and shellfish are caught each year, causing serious problems in the environment. So that the utilization of fish bones skeletons waste will decrease environmental pollution of our country and increase the national economy. The Preparation of the scaffolds from biogenic resources qualified them for bone formation. The prepared scaffolds are characterized by their good mechanical efficiency. The preparation method included three essential steps: First step: 1. Preparation of pure and fine hydroxyapatite powder having a grain 50 nm from natural resources such as fish bone skeletons by thermal treatment. 2. Formation of highly porous hydroxyapatite scaffolds prepared from fish bone skeletons having a grain ) 974.1 nm to 3.112 𝛍 m) using Polymeric Sponge Method Second step: Reinforcement of hydroxyapatite scaffolds by coating with PCL to produce HA/ PCL (2, 4, & 6 %) for different time intervals (5, 10 and 15 min) scaffolds by the method of polymer impregnating to produce HA scaffold coated with PCL composites. So, the best socking was HA/ 4 % PCL for (10 min). The chemical structures for the samples were studied using (FTIR). For morphological structure, The SEM microscopy associated with (EDX) was used in order to determine the shape of the obtained components, the degradation behavior at different time intervals, and the weight loss %, after immersion in double distilled water for 1 month, were recorded. Third step: HA& HA/ 4 % PCL scaffolds were implanted in femur bone defects of fortieth rats to evaluate its ability to regenerate bone tissue. The other twenty rats were not subjected to surgery. They served as a control group for comparison regarding blood analysis. - Biological studies for safety evaluation of implanted materials It is important to study the biological behavior of any materials implanted inside the animals and to estimate their safety evaluation to be used as bioceramics. The blood samples were taken after 2 and 6 months of experimental study, these animals was divided into three groups:- 1. Control group This group includes 20 normal control rats. Hydroxyapatite scaffolds treated group This group contains 20 normal albino rats treated with HA scaffolds. 3. Hydroxyapatite / 4 % PCL scaffolds treated group. This group having 20 normal albino rats treated with HA / 4 % PCL scaffolds. The blood analyses were carried out as the following: -Liver and kidney functions tests: Statistic analysis showed normal liver and kidney function as compared to the control group. - Tumor markers and Free radical biomarker. No carcinogenic effect and no oxidative effect over the animals as compared with the control group. - Inflammatory effect No inflammatory effect. |