الفهرس 
OXIDATIVE STRESS AND ITS RELATION TO VARIOUS DISEASES
DefinitionOnly 14 pages are availabe for public view
 |  | from | 145 |  |  |
| | | from | 145 | | |
Abstract
In this study, we were concerned to measure serum levels of
NO and MDA as indicators of oxidative stress in known
patients with pediatric SLE to study their relationship to the
disease activity.
We conducted this study on 20 children with pediatric
onset SLE attending the Pediatric Allergy and Immunology
Clinic, Children Hospital, Ain Shams University.
Clinical evaluation of SLE patients with assessment of
the disease activity using SLEDAI was done with special
emphasis on age at onset of SLE, duration of the disease,
medications given, body organs affected and other associated
co-morbidities. Clinical examination included assessment of
blood pressure and edema and physical examination of
different systems .
Laboratory investigations included CBC, ESR,
immunological markers (anti-dsDNA, C3, lupus anticoagulant
and anticardiolipin Abs), kidney function tests (serum
creatinine, creatinine clearance and 24 hours’ urinary proteins)
and lipid profile (T. Cholesterol, TG, HDL and LDL).Serum NO and MDA levels were measured by ELISA in
pediatric SLE patients.
The present study revealed that serum NO (Mean 249.31,
± SD 35.93) and MDA (Mean 14.64, ± SD 3.74) levels were
significantly higher among SLE children in comparison to
controls (serum NO (Mean 97.85± SD 12.87),serum MDA
(Mean 2.16± SD 0.59))
There was a significant positive correlation between
SLEDAI grades and serum NO & MDA levels. There was a
significant positive correlation between number of
exacerbations and NO serum levels.
Children with associated comorbidities (hypertension)
had significantly higher levels of serum MDA.
Children with abnormal lipid profile (high LDL
cholesterol and low HDL cholesterol) had significantly higher
NO & MDA serum levels.
Children with proteinuria had significantly higher levels
of serum NO.
Patients with high serum antibodies (Anti DNA antibody
and Anticardiolipin IgM & IgG) had higher levels of serum
NO.Patients with high serum anticardiolipin IgM & IgG had
higher levels of serum MDA.
Diet control had a significant impact on serum NO &
MDA levels. Children without diet control had significant
higher NO & MDA serum levels.
Immunosuppressive therapy had no significant impact on
serum NO & MDA expression.In conclusion, elevated serum NO &MDA levels in
children with SLE suggested that systemic NO&MDA
upregulation were induced by lupus disease activity. Further
studies on a wider scale are needed to strengthen the findings of
this study, to validate the usefulness of those biomarkers as
markers of lupus activity and for better understanding of their
role as predictors of disease severity and outcome.