الفهرس 
Materials and MethodsOnly 14 pages are availabe for public view
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Abstract
The activity of a-glucosidases enzymes synthesized by I 00 strains of yeast fungi isolated from
Egyptian soils were qualitatively examined. Quantitative assay test showed that isolates No. 24,
27, 36 and 96 were the most active producers. These were characterized and identified as
Wicherhamia fluorescens, Schwanniomyces accidentalis, Pichia strasbuigensis
and Hensenula anomala respectively.
Studies on the nature of a-glucosidases enzymes clearly . indicated that these enzymes
were constitutively synthesized by these four yeast fungi. In addition, factors affecting
a-glucosidase
activity were also determined.
The nutritional requirements and growth conditions required for maximum a-glucosidases production
by the most active species (Hansenula anomala) were determined.
In addition, various treatments to release good yield of the active enzymes from the yeast cells
were carried out.
On the other hand, the enzymes were purified by ammonium sulphate, ethanol and acetone
precipitation and gel sephadex G-
100 filtration. Furthennore, some physiochemical and biochemical
properties of the purified enzymes were investigated.