الفهرس | Only 14 pages are availabe for public view |
Abstract The main goal of the present study was the analysis of ~-lactamase production in S.aureus isolates from - humans and bovine mastitis and the association of lactamase production to methicillin and other antibiotic resistance.B-lactamase production was assayed using Cefinase disk method and MRSA was detected using Cefoxitin sensitivity test. The association of ~-lactamase production and basic test results as carotenoid pigment and hemolysin production was analyzed. The obtained data revealed that, the percentages of J3-1actamase production by S.aureus strains isolated from bovine milk (cows and buffaloes) were 63.63% and 80%, respectively. Meanwhile, 13- lactamase production by S. aureus strains isolated from human cases (urine, nasal swabs and blood) was 71.43%, 51.6% and 66.7%, respectively. The results obtained proved that, 59.09% and 60.00% of S.aureus strains isolated from bovine milk (Cows & buffaloes) were Methicillin-resistant, respectively. While 100%, 70.97% and 50.0% of Scaureus isolated from human cases proved to be Methicillin-resistant. respectively. Of 86 Surureus isolates identified 55 (63.95%) produced ~-lactamase of these 39(70.91 %) proved to MRSA and 16(29.1 %) Multi drug resistant (MDR).The association of r3-1actamase production and MRSA and MDR was discussed. The main goal of the present study was the analysis of ~-Iactamase production in S.aureus isolates from humans and bovine mastitis and the association of ~-lactamase production to methicillin and other antibiotic resistance. Overall, there is clear evidence in this work that p-lactamase producing Svaureus and MRSA can be transmitted between humans and animals. |