الفهرس 
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Abstract
In the present study, sixty patients from El-Houd El-Marsoud hospital suffering from tinea unguium and onycomycosis were tested in the period from October 2003 to Oct.2004. Their age ranged between 20 and 60 years, with a mean of 14.1 years and standard deviation of 10.8. These cases include: foot infection which is the major type of infection followed by nail infection, hand infection and scalp infection. All the specimens were collected in clean Petri-dishes, a portion of the specimen was used for direct microscopic examination after clearing the preparation with KOH (10-20%), and another portion was used for culture on SDA and left at temperature 25-30 C° to be observed after 2-5 days and for as long as 4 weeks. The texture, topography, pigmentation and the rate of growth of the different isolated fungi were observed. In this study of dermatophytosis (50%) of cases were positive by direct microscopic examination using KOH, 53.3% of cases gave positive cultures. The prevalent organisms isolated were: C.albicans (46.9%), followed by T.mentagrophytes (21.9%), A.niger (18.8%), and the least was A.flavus (12.5%). The effect of solar simulator light on the tested fungi ranged from death to partial inhibition. The inhibition of spore germination of the tested fungi was significantly increased when the solar simulator light was applied with different fluence rate, different exposure time and treatment with different concentration of crystal violet. The effect of sun light on the tested fungi ranged from death to partial inhibition. There was a significant increase in percentage inhibition of spore germination when the sun light was applied with different exposure time and treatment with different concentrations of crystal violet. The effect of He-Ne laser irradiation on the tested fungi ranged from death to partial inhibition when increasing doses of laser light as well as increasing concentrations of photosensitizer in order to achieve the optimal dose of laser light with optimal photosensitizer concentration which was required for fungal killing. In Conclusion, The use of solar simulator light, sun light or He-Ne laser in the presence of crystal violet as photosensitizer have resulted in inhibition of spore germination of some species causing tinea unguium and onychomycosis in vitro. This study needs to be followed by in vivo further studies in order to evaluate the possible use of this technique in treating patients.