الفهرس 
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Abstract
Canine infectious tracheobronchitis (kennel cough) caused by CAV-2 is one of the most prevalent respiratory infections of dogs. Kennel cough characterized by frequent dry and coughing, high morbidity but low mortality rate. In this study, nasal and ocular discharge samples have been taken from 59 dogs from the veterinary clinics of pet animals in Cairo, Egypt during 2013 - 2014. It showed fever, oculonasal discharge, conjunctivitis and dehydration. Suspected virus was identified by chromatographic immunoassay, the result was positive for CAV in only 7 samples. Then the virus from positive samples was passaged for 3 blind passages in Vero cell then subjected to FAT and haemagglutination tests, the results indicated that the virus was most likely to be present in the respiratory tract. The virus was inoculated onto MDCK, Vero, BHK21 and MDBK cells for 10 passages. The Cytopathic effect (CPE) appeared and MDCK cells were the most susceptible cell culture where they gave the highest infectivity titre.