الفهرس 
AcknowledgmentOnly 14 pages are availabe for public view
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Abstract
Silver nanoparticles (AgNPs) are widely used in industrial, daily life, healthcare and medical products such as water purifiers, toys, sunscreen, wound dressings, urinary catheters and bone prostheses. So we need more studies about AgNPs adverse effects. in this study we discuss the effect of AgNPs (with mean particle size 20 ± 1.2 nm) and comparing it with same dose of AgNO3 at the cell level by using primary isolated rat hepatocyte. we highlight the AgNPs cytotoxicity by assessing cell viability through trypan blue exclusion test ,determination of enzymatic leakage percent and mitochondrial damage through analysis of ALT, LDH, AST enzymes release, assessment of oxidative stress by determination of reduced glutathione and lipid peroxides, examination of histopathological changes under light and electron microscope, and AgNPs genotoxicity through detection of DNA damage by COMET Assay and apoptosis by DNA Fragmentation Test. Results reveal that cell viability and glutathione level in groups treated with AgNPs and AgNO3 significantly decreased from control group, but lipid peroxidation level and enzymatic leakage increased in silver treated groups either in nano or micro form compared with control group, in addition to presence of DNA damage and apoptosis appear in COMET assay and DNA Fragmentation Test respectively, and finally we found alteration in morphology of all treated cells from control one through light and electron microscopes, We summarize these effects on the cell which are helpful for risk assessment of AgNPs usage in the future.