الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Biotechnology approaches can be used to enhance the level of carotenoids production by natural organisms. Usage of recombinant DNA techniques is one way to increase the production of carotenoids in biological systems. Many algal strains have been developed by classical strain improvement methods to produce various types of carotenoids. However, the carotenoid productivity of the generated organisms is still low compared to chemically synthesized carotenoid. The carotenoid biosynthetic pathway is amenable to manipulation by recombinant DNA techniques since most carotenoids share a common precursor. Phytoene synthase(PSY) is one of the crucial enzymes involved in carotenoids biosynthetic pathway. The coding sequence of the cyanobacterial gene PSY was cloned into expression vector and transformed into Dunaliellaparva cells. RT-PCR analysis of PSY mRNA transcripts showed that the PSY gene is functionally expressed in the transformed algal cells. Transgenic D. parva cells showed enhanced level of carotenoids compared to control cells when the algal cells were allowed to grow on variable types of agricultural wastes. Transgenic D. parva showed the highest level of carotenoids and antioxidant activity. These results provide novel strategies to develop genetically modified microalgae with enhanced level of carotenoid production.