الفهرس 
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Abstract
A total number of 1350 ovaries of pregnant and non pregnant dromedary camels slaughtered at cairo slaughter house were collected during the period October 2011 to January 2014. The oocytes were collected by aspiration of the ovarian follicles of 2-8mm or by slicing technique. The selected oocytes were matured in vitro in TCM-199 supplemented with either cysteamine, caffeine, FSH, LH, PMSG, IGF1, EGF then incubated at 39°C in high humidity air with 5% CO2 for 30 hours. In vitro matured oocytes were inseminated by in vitro capacitated epididymal sperm obtained through flushing of epididymal sperms of mature male camels slaughtered at cairo slaughter house , the oocytes and epididymal sperms were incubated together in F-TALP medium for 24 hours in the same conditions of maturation.The presumptive zygotes were cultured in synthetic oviductal fluid medium and incubated at 39°C in 5% CO2 for 6-7 days, and examined for cleavage, morula and blastocyst. The results indicated that the average number of recovered oocytes was significantly higher (P<0.05) using slicing method (2.82) than aspiration (1.83). The frequency of grade I oocytes was significantly higher by aspiration than slicing (50.1% vs. 22.6%).Using of 100um cysteamine on the maturation medium resulted in higher maturation and fertilization rates(64.0% and 52.2%), also using of 10 mm caffeine addition to the maturation medium at last 6 hours of maturation resulted in higher maturation and fertilization rates (70.5 and 58.9%).Combined FSH and LH in the maturation medium resulted in higher maturation and fertilization rates(72.0% and 58.1%), also combined IGF1 and EGF resulted in higher maturation and fertilization rates (71.7% and 55.9%).Oviductal cell monolayer enhances cleavage, morula and blastocyst (41.4%, 19.4% and 10.9%) than granulose cell monolayer (30.6%, 10.2% and 3.8%).