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Abstract Colibacillosis in chickens caused by avian pathogenic E.coli still being a major disease problem that affecting all poultry flocks; broilers, layers and breeders allover the world and specially in Egypt due to many factors related to management of poultry industry at all. Many factors share in Colibacillosis arising and make the infection so economic. This study was planned to investigate virulence factors among APEC for the detection of virulence chromosomal genes and for studying its multidrug resistance profile to correlate the genes occurrence and drugs activity among chicken flocks: broilers, layers and breeders in Egypt. Multiplex PCR technique was applied to detect the virulence chromosomal genes that may accompanied with its virulence activity. The results showed that: Regarding to the results of incidence of L.F. isolates (63%) showed positive E.coli isolates while (37%) were non E.coli isolates Regarding to the uptake of congo red dye between the isolates (78.2%) were positive and were considered invasive and (21.8%) still white colonies and considered non-invasive. The results obtained by application of disc diffusion test to detect the resistant and sensitive antibiotics; the results showed that all isolates were resistant to rifamycin and were highly resistant to streptomycin, erythromycin and doxycycline in a percentage of |