الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Stroke is a leading cause of death and permanent disability in adults worldwide. Intravenous recombinant tissue plasminogen activator (rt-PA) is the only FDA-approved treatment for administration within 3 hours of onset of acute ischemic stroke.
Among the mechanisms involved in stroke is the activation of phospholipases with subsequent release of arachidonic acid (AA) and docosahexanoic acid (DHA). AA yields eicosanoids which are implicated in the induction and maintenance of the acute inflammatory responses while DHA is a substrate for the biosynthesis of resolvins and protectins that have both anti- inflammatory and pro-resolution activities.
In this study, the effect of delayed administration of DHA (IV
28 mg/kg, after 5 hours) was investigated against brain damage following permanent or reversible ischemia induced experimentally in mice by middle cerebral artery occlusion (MCAO).
Treatment with DHA showed the following results:
- Significant reduction in the infarct area and infarct volume following both permanent and reversible ischemia but with higher reduction in reversible rather than permanent MCAO.
- Significant reduction in brain edema following reversible ischemia but with no effect following permanent MCAO.
- Significant improvement in the sensorimotor dysfunction on neurological score and corner test after reversible but not permanent MCAO.
- Significant reduction in leukocyte infiltration following the reversible ischemia while no significant reduction was
observed following permanent ischemia.
- Significant reduction in neuronal degeneration (Fluoro-Jade fluorescence) following reversible but not permanent MCAO.
- Significant reduction in NF-κB activity following reversible
but not permanent MCAO.
- Significant reduction in 15-LOX-1 level following both permanent and reversible MCAO.
Based on the previous findings, the following can be concluded:
1) Reversible MCAO can cause higher damage and infarction than permanent MCAO.
2) Reversible MCAO can cause more infiltration of peripheral leukocytes plus higher induction of NF-κB activity than that caused with permanent MCAO.
3) Reversible MCAO has more deleterious effect on sensorimotor function than permanent MCAO.
4) Both permanent and reversible MCAO caused elevation in the level of 15-LOX-1 and 15-LOX-2.
5) DHA treatment had more influence in reducing sensorimotor dysfunction, infarct area, infarct volume, leukocyte infiltration, brain edema, NF-κB activity, neuronal death and
15-LOX-1 level following reversible rather than permanent
MCAO.