الفهرس 
AbstractOnly 14 pages are availabe for public view
 |  | from | 139 |  |  |
| | | from | 139 | | |
Abstract
In this study 500 dead or ailing layer were examined clinicaly chickens from differnet farms in El-Sharkia, El-Kaliubia, El-Fayoum, El-Giza, El-Menia, El-Behira and El-Monofia suffering from signs of infectious coryza. The examined chickens showed severe respiratory signs as well as a marked DROP in egg production. Postmortem examination revealed conjunctivitis, infra-orbital sinusitis and nasal discharge. Sinus exudate samples were taken and subjected to extensive bacteriological and molecular examination, which included colony morphology, biochemical identification, animal pathogaenicity in embyonated chicken eggs and molecular characterization. The result was isolation and identification of Avibacterium paragallinarum using PCR primers specific to the microorganism was used for confirmation of the biochemical results. Serotyping using PCR based protocol resulted in 60% of isolates were Page’s serovar A and 40% of the isolates were Page’s Serovar C.
An experiment to use the fresh yeast extract as a substitute to the NAD in the media was done using isolates from this study and 3 standard strains and resulted in proving the ability of Avibacterium paragallinarum to grow without NAD on media that contained only fresh yeast extract and chicken serum
A trial to produce a coloured antigen for rapid diagnosis of infectious coryza has been conducted but unfortunately it was unsuccessful the formation of auto agglutination in the negative control samples.