الفهرس | Only 14 pages are availabe for public view |
Abstract The present work was carried out at the Biotechnology Laboratory, Faculty of Veterinary Medicine, Kafrelsheikh University. The present study is the first Egyptian report on polymorphisms of GH, GHR and GHSR genes and the association between their SNPs and eight body weights in seven Egyptian chickens (Fayoumi, Dandarawy, Dokki-4, Inshas, Mandara and El-Salam and Rhode Island Red) 26 birds from each breed. In this study, blood and muscle tissue samples were collected from seven Egyptian chickens and then DNA and RNA were extracted. A set of novel SNPs were detected. Of these SNPs, G92A SNP in the second intron of cGH gene and homozygous genotype GG of this SNP was associated with higher eight week body weights. A novel T225C SNP was detected in the fourth intron of cGH gene. A synonymous A93C SNP was detected in exonic region of chicken GHR gene with no observed change in amino acid. A novel T40C SNP was detected in the first intron of GHSR gene in (Dokki-4, Inshas, Mandara, El-Salam and Rhode Island Red) and not detected in Fayoumi, Dandarawy. . This study may be practical way for genetic improvement of Egyptian native chickens. |