الفهرس 
Nutritive value of MushroomsOnly 14 pages are availabe for public view
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Abstract
Student Name: Lena Ahmed Saleh Al-Faqeeh
Title of the thesis: Extraction, Purification and evaluation of some
antitumor compounds from Pleurotus ostreatus
Degree: Master of Science (Microbiology)
A factorial design L18 (21×11×36) was used to construct 18 media with
different composition and condition, in addition to modified medium containing all
the optimum factors for maximizing the proteoglycan production by the edible
mushroom Pleurotus ostreatus. Media 16, 18 and modified were selected for mycelial
proteoglycan study, whereas fruiting bodies grown on rice straw were chosen for
fruiting bodies proteoglycan study. Complete purification scheme using ion exchange
on DEAE- cellulose and gel filtration chromatography on sephadex G-100 revealed
the presence of three peaks in fruiting bodies proteoglycan (FF1, FF2 and FF3) and
one main fraction in mycelial proteoglycan (MF1). The FTIR revealed spectra of β-
glucosidic bond, OH, C-O and C-O-C stretching bonds and spectra peaks of amide І,
П and Ш. The HPLC analysis of the monosaccharides composition of Pleurotus
ostreatus proteoglycan indicated the presence of arabinose, fructose, mannose,
glucose and galactose in varing ratios among pure fractions. The amino acids analysis
of proteoglycans showed the presence of 18 amino acids with leucin being of highest
quantity. In vitro anticancer assay of the proteoglycans of P.ostreatus revealed its
potent anticancer activity against cervical carcinoma (HELA), breast carcinoma
(MCF7) and larynx carcinoma (HEP2) and to less extend on liver carcinoma
(HEPG2) cell line while colon carcinoma (HCT116) was resistant. The crude extracts
were more efficient than the pure fractions. In vivo assays using head and neck cancer
bearing mice indicating that the pure fractions were more necrotic to cancer cells than
the crude extracts. For rapid prediction of cancer advancement and healing in head
and neck bearing mice, three biological markers were assayed which are plasma
catalase, Red blood cells glutathione peroxidase, plasma lipid peroxidase and three
elements which are Zinc, Copper and Selenium. Measurments of these markers in
head and neck bearing mice treated with P.ostreatus proteoglycans predicted a sign of
cancer inhibition or healing.
Keywords: Pleurotus ostreatus, cancer disease, anticancer activity, proteoglycan
compounds, cancer markers