الفهرس 
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Abstract
Because of the economic importance of equine in Egypt especially the pure Arabian horses sharing in racing and equestrian events that support the trade exportation must be completely healthy and free from any viral or bacterial injections disease
Through the control of it using vaccination,
The present study was designated to prepare and evaluate inactivated equine influenza vaccine with three different adjuvants .
with the following investigation point .
1- Preparation of three types of inactivated equine influenza vaccine against subtypes
A) EI inactivated vaccine adjuvanted with montonied oil ISA 206 .
B) liquid inactivated virus vaccine adjuvanted with sopnin.
C) liquid inactivated virus vaccine adjuvanted with mycobacterium phlei extract
• the virus suspension was prepared from local isolate of EI-2 (A/equi -2/alex/1-2008),which propagated in ECE (9-11 days old for 5 passage ).
• - complete virus inactivation was achieved at 37 Co after 18 hours with final concentration at 0.003 M at BEI.
• The inactivated virus suspension was proved free from residual injective virus in ECE 9-11 days old.
• Addition of each adjuvants to the inactivated virus fluid :
a- Addition of inactivated virus fluid to montanide oil (W/W)
b- Addition of inactivated virus fluid to saponin 2mg/dose
c- Addition of inactivated virus fluid to myco bacterium phlei non specific immuno stimulant to inactivated virus fluid 100mg dose for each horse contain 2 mg.
2 – evaluation of the prepared vaccine :
a- sterility: the final product of the vaccine was tested for its sterility using different bacterial media and they were free from any bacterial or fungal contamination
b- Preliminary study of the immunogenic potency of the prepared vaccines in 4 groups of G.Pigs show that : A group of guinea pigs which inoculated with montanied oil ISA 206 gave mean titer of HI antibody (2457) while the 2nd group inoculated with soponin is 973 while the 3rd group inoculated with mycobacterium phlei extract is 192 and the 4th group which left as a control gave negative results.
- from the results of G.Pigs inoculations it is clear that the inactivated montanide oil ISA 206 adjuvant vaccine is more immunogenic then other types of vaccines.
c- Keeping quality of the prepared inactivated EI vaccine adjuvanted with montanide oil ISA 206 vaccine was evaluated in G.Pigs – the vaccine could be kept at 4 Co for more than one year and kept at 25-28 Co ) for 6 months only with out loosing it’s immunogenic properties
- while the other group which inoculated with inactivated EI vaccine adjuvanted with soponin could be kept at 4 Co for one year and can’t be kept at room temperature.
d- Safety test of the 3prepared vaccine which inoculated separately in horses showed No up normal local or systemic reaction following the initial or boostering inoculation.
E- The immune response of susceptible horses inoculated with prepared inactivated EI vaccine were traced until 10 months post inoculation showing that the best immuno response comes from both those adjuvanted with montanide oil ISA 206 and saponin while those adjuvanted with myco bacterium phlei gave rapid cellular immune response and the Peak at 10th days
Conclusion
it is advisable to use EI inactivated vaccine either adjuvanted with montanide oil ISA206 (10 months ) or saponin ( 8months ) with caution that the keeping quality of that adjuvanted with saponin can kept at 4 Co for one year and can’t kept at room temperature while those that adjuvanted with oil can be kept at 4 Co for over one year and kept at room temperature for 6 months without effect on its immuno genic characters.