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Abstract The radiation exposure one of the risk factors of cancer, especially in the field of medical diagnostic and treatments, in addition to the daily exposures of radiation; industrial, large number of atomic radiation, the increased spread of car exhaust, smoking, stress and unhealthy eating. Also, the consequent increase in the oxidation process in the body and the production of free radicals, leading to disruption of operations, damage of vital tissues and the occurrence of mutations, which in turn lead to organ failure and then death or a growth of abnormal cells or so-called cancer. The body’s daily compensation tissues and damaged cells, where Allah creating in our bodies the necessary defensive methods, which resist harmful these radicals and protect us from dangers, such as enzymes, antioxidants, including (glutathione, glutathione peroxidase, super oxide dismutase, and catalase) and the immune system. But, if the speed of the formation of free radicals more than the body’s energy defense speed, the body is unable to defense and protect the tissue from the danger. Therefore, the need to identify substances that have the ability to curb and protect the tissues from damage induced by free radicals is a great need in the fields of radiation exposure, especially in the medical field. The natural compounds of plant especially phenolic compounds, flavonoids, some minerals as zinc and some vitamins of the most important compounds that play a major role in radioprotectors field specially in radiotherapy. Para-coumaric acids and caffeic acids are natural secondary metabolite compounds to phenolic cinnamic acid, which are used in this area largely, it act as antioxidant, immune system stimulate and anti-cancer. However, the effective impact of 149 these compounds and the natural compounds generally are little, because the molecules size are large, metabolite slowly and poorly transported across the cell membrane, so the number of molecules that reaching the cell to perform effective role are small and also it causes some side effects as a result of the accumulation of molecules on the cell membrane, in addition to the acids have some adverse effects on the stomach and the process of absorption. Therefore, the study aimed to assess the potential role of protective and therapeutic impact of Para-coumaric acid and caffeic acid in nano particles form, removal the acid and adding zinc metal to become the new compound name; Zinc Coumarate Nano Particles (ZnCoum.NPs) and Zinc Caffeiate Nano Particles (ZnCaf.NPs), were given to rats by intraperitoneal injection at a concentration of 5 mg/kg and 15 mg/kg body weight respectively, for 7 successive days, post irradiation for (ZnCoum.NPs + ZnCaf.NPs) and [(ZnCoum.NPs + ZnCaf.NPs) + Irradiated] groups and for 30 successive days for (ZnCoum.NPs + ZnCaf.NPs), [(ZnCoum.NPs + ZnCaf.NPs) + Irradiated] and [Irradiated + (ZnCoum.NPs + ZnCaf.NPs)] groups, against whole body -irradiation with a dose of 3Gy, 4 times, every week up to 12 Gy, through the study of biochemical changes that occur in the activity of liver enzymes, antioxidants, oxidation of fat, and kidney function, and the concentration of free radical, DNA fragmentation, CD4 cell and CD8 cell percent, phases of cell cycle and histologic changes of liver cells. Animals were divided into five main groups, each group divided into four subgroups according to fractionated doses. Group I (24 rats): (Control) Non-irradiated, non-treated normal control rats. 150 Group II (24 rats): (ZnCoum.NPs + ZnCaf.NPs) Non-irradiated, rats treated with Zinc coumarate nano particles (ZnCoum.NPs) and Zinc caffeiate nano particles (ZnCaf.NPs). Group III (35 rats): (Irradiated group), Rats were exposed to whole body -radiation, 3Gy every week for four weeks. Group IV (24 rats): [(ZnCoum.NPs + ZnCaf.NPs) + Irradiated], Rats were injected with ZCoNPs + ZCaNPs on seven consecutive days, then subjected to whole-body gamma radiation 3Gy every week for four weeks, 20 minutes after the last dose of ZnCoum.NPs + ZnCaf.NPs injection. Group V (24 rats): [Irradiated + (ZnCoum.NPs + ZnCaf.NPs)], Rats were subjected to whole-body -radiation, 3Gy every week for four weeks, then injected with ZnCoum.NPs + ZnCaf.NPs. The results of this study explained that the dosing rats by gamma rays led to the decrease evident in the percentage of body weight compared to normal control, this has been attributed to the toxic effects of gamma rays on the organs of the body but with the treatment with ZnCoum.NPs and ZnCaf.NPs, it can protective animals from damage compared to normal control. It was noted that the level of lipid peroxidation in the blood of rats exposed to gamma rays has increased significantly compared to normal group accompanied by a decrease in the level of glutathione and glutathione peroxidase as well as superoxide dismutase and catalase in blood, and white and red blood cells, platelets and the level of hemoglobin in the complete blood count, also found that there is an increase in enzymes activity of alanine aminotransferas, aspartate aminotransferase, the level of albumin, creatinine, urea,. |