الفهرس 
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Abstract
Biofilm is a bacterial lifestyle widespread In microbial world and is a mawr concern In health care. Particularly relevant are the medical device related infections caused by StaphylococcLis allrellS and S. epiderlllidis grown in biofilm (Bestul and Yandenbussche, 2005).
Staphylococci are common cause of hospital-acquired infection and
hiofilm IS one of the important microbial virulence factors found In <.larhylococci (Cramton and Gerke 1999; Gotz 2002). Biofilm consists of
multilayered cell clusters embedded III a matrix of extracellular
polysaccharide, which facilitate the adherence of microorganism.
Staphylococci are commensal on human body surfaces and are also known to coloni7c the biomedical devices such as peripheral IYO (Frebourg and Lcfebvrc, 2000, and Gotz et aI., 2002).
Early detection and management of potentially pathogenic staphylococci can be one of the essential steps tOvvards prevention and management of device-associated nosocomial infections. There IS also a need to evaluate a simple method lor detection of biofilm producers. Biofilm production can be a marker of virulence. which can be detected phenotypically. We studied the biofilm producing potential of staphylococci strains from patients III a pediatric ward.
Congo red agar (CRA) and one quantitative microtiter plate (MTP) methods were uscd for detection of biofilm production. The accuracy of eRA method for detection of biofilm was also evaluated by using MTP method as gold standard.
In our study reported that CR is a specific method for’ the d~tection or biolilm rroducers. Most of the studies used either MP quantitative or eRA qualitative method for biofilm detection. Earlier some studies proposed CR/\ kst as an alternative to MTP test for screening staphylococcal isolates for biolilm production. Biotilm producers produce black to almost black color col()llic~ ami Ilon-pruducers form pink to Bordeaux colored colonies on CRA. It is known that Congo red can directly interact with certain polysaccharide forming colored complexes