الفهرس | Only 14 pages are availabe for public view |
Abstract 40- kOa protein was purified from concentrated culture supernatant of C. pseudotuberculosis using hyDROPhobic interaction chromatography. Four different vaccines were investigated for their efficiency in protecting sheep against C. peudotuberculosis. Vaccine composed of purified 40-kOa protein, vaccine composed of recombinant mutant phospholipase- D (PLD), vaccine composed of purified 40-kOa protein plus recombinant mutant PLO, and Vaccine composed of formalin-killed C. peudotuberculosis plus recombinant mutant PLD. The present study concluded that the combined vaccine composed of 40- kOa protein plus recombinant mutant PLO succeeded in stimulating both humoral and cell mediated immune response and provided a high level of protection against CLA in sheep (92%). |