الفهرس | Only 14 pages are availabe for public view |
Abstract The genetic diversity of twelve Egyptian hexaploid wheat (Gemmiza 7, Gemmiza 9, Sids1, Giza 168, Gemmiza 10, Giza 170, Sakha 93, Sakha 94, Sakha 61, Sakha 69, Gemmiza 3 and Gemmiza 5) cultivars were investigated based on eleven morphological characters (Plant height, flag leaf area, number of days to flowering, number of days to maturity, length of main spike, weight of main spike, number of spikelets / spike, number of grains / spike, weight of 1000 grain, number of spikes /m² and grain yield / plot), peroxidase activity, seed storage protein electrophoresis and RAPD-PCR markers and the inter cultivars relationships were studied based on the analysis of the morphological, seed storage protein electrophoresis and RAPD markers. The peroxidase activity of the studied cultivars produced five bands (Pex1,2,3,4,5), three out of them were monomorphic and the other two were polymorphic. The SDS-PAGE analysis of water soluble proteins produced 20 bands ranging from 2.9 to 212 KDa, five out of them were common bands, while the other 15 bands were polymorphic. The buffer soluble seed storage protein electrophoresis produced fourteen bands ranging from 6.2 to 78.6 KDa. Six out of them were common bands, while the other 8 bands were polymorphic. One hundred and fifteen RAPD-PCR markers were resulted from fingerprinting the twelve wheat cultivars under study using 11 arbitrary primers, 88 bands out of them were polymorphic and the other 27 were monomorphic. Comparison of the dendrograms resulted from the RAPD-PCR data analysis with those resulted from both morphological data analysis and protein data analysis, it was appeared that, all dendrograms were relatively similar. The results of this study showed that, morphological traits, peroxdase activity, seed storage protein electrophoresis and RAPD-PCR markers have been succeeded in cultivar identification by determination of the genetic diversity of wheat genotypes under study and addressing the genetic relationships among them. |