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Abstract The present investigation was carried out an 105 native buffalo-calves, 15 days to 6 months old of both sexes. The buffalo-calves were classified according to feeding regime into: Pre-weaning stage included 39 calves and Post-weaning stage included 66 calves of both sexes. Another classification according to age was 15 days old (19 calves), one month old (6 calves), two months old (14 calves), three months old (16 calves) and 4 to 6 months old (50 calves). Venous blood samples were taken anaerobically from each animal by syringe whose dead space had previously filled with 1000 u/ml sodium heparin and preserved in ice-water bath till time of analysis. Blood gases measurements were performed by CORNING pH-blood gas analyzer model 168. The measured parameters of blood gases and acid-base balance, were hydrogen ion concentration (pH), carbon dioxide tension (Pco2), oxygen tension (Po2) bicarbonate (Hco3), total carbon dioxide tension (Tco2) and base excess (B.E). The mean values of pH, Pco2, Po2, Hco3, Tco2 and B.E for the buffalo-calves at the pre-weaning stage were 7.362 + 0.05, 40.839 + 4.9, 33.649 + 4.5, 23.541 + 3.6, 24.849 + 3.8 and -0.9 + 4.3 respectively. Also the mean values of the same mentioned parameters for blood gases and acid-base balance for the buffalo-calves at the post-weaning stage were 7.361 + 0.05, 49.559 ± 3.9, 30.125 ± 6.7, 28.02 ± 2.53, 29.57 ± 2.58 and 2.13 ± 2.9 respectively. The values for blood gases and acid-base balance showed significant difference between pre-weaning and post-weaning stage expect for the PH. The measured parameters revealed non significant difference between male and female animals within the same period either the pre-weaning or post-weaning stage. On the other hand the age factor had an effect on values of blood gases and acid-base balance, where in per-weaning stage the data showed non significant difference for PH, PCO2, HCO3, and B.E. while significant difference was observed for PO2, and TCO2 at the same time. In post-weaning stage the results revealed non significant difference for PH, HCO3 and B.E. while significant difference were seen for PCO2, PO2, and TCO2. |