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Abstract -101- SUMIVlARYAND CONCLUSION The present invesiiigation was carried out to determine the effect of growth promoting substances,i.e., GA 3 and NAA and microwltrients applied as a foliar spray individually and in combination at different periods on the oehaviour of roselle growth. Therefore, two field trials were conducted at Bahtim Agricultural Experimental station,Agricultural Research Center using roselle variety,namely’ Balady,in 1979 and 1980 seasons. The design of the experiments were complete randomized block with three replications. Plots were sprayed four times with growth promoters and micronutrients according to the treatments during growth period. Treatments of the first season include GA3 100 ppm,NAA lOOppm and micronutrients, i.e• Fe,Zn,Cu,Mn,(Fe + Zn+ Cu + Mn) and their combinations. However, treatments of ”the second season includedGA 3 (50 and 100 ppm),NAA (25 and 50 ppm),micronutrients (Fe + Zn + Mn + Cu + B + Mo) and their possible combinations. The main trend of results can be summarized as follows: 1- NAA, GA 3 at the rate of 100 ppm increased plant height during the growth periods of roselle. NAA + GA3 increased plant heie~t at earlier period of growth while -- -------~-- - ---- - ---------------- descreased it at later :stages. spray of Fe or Cu increased plant height,however,Zn,Mn and (Fe + Zn + Cu + Mn) decreased it. 2- Stem dry weight increased gradually with advancing plant age, reached its maximum at 125 days after sowing then decreased through maturity.The grand p~riod of stem growth occurred during the fourth period (100-125 days after sowing). 3- GA 3 and NAA favoured the accumulation of stem dry weight. The addition of micronutrients inhibited the promotive effect of GA3 and NAA. 4- Leaves dry matter increased gradually to reach its maximum when plants reached the age of 125 days,then dropped. Both growth substances and micronutrients had no effect on leaves dry matter at earlier periods of growth but reduced it during the second period (75 days after sowing) while enhanced such dry matter accumulation through later periods of growth (100-125 days). 5- Dry weight.t rej)roductive organs was increased.by GA 3 ’ NAA and micronutrients. NAA at 25 ppm treatment had the highest dry matter of such organs. 6- Dry matter accumulation in different fruit parts of roselle was annanced by the use of GA3, NAA and micronutrients. __ .___ __ _0’ __ --- ---- - --- _. -- - ---- - - ------- ----------~ -103- 7- Dry matter accumulHtion of the whole plant was favoured by GA 3 ’ NAA and micronutrients application especially at later stages of roselle growth. 8- Leaves comprise d/’5 ~ of total dry matter of whole plant at earlier stages of growth (50 days after sowing). Then decreased gradu8.11Y to rea.chits lowest value through the reproductive period. 9- Dry matter accumulation in stem increased gradually on account of the decr1aase in leaves dry matter. 10- Growth promoting ~ubstances and micronutrients affected the nature of regulating mechanism of dry matter distribution and accumulation in roselle plant. 11- Both growth sUbE:tances and micronutrients had a significant effect on bast fiber formation, especially at later periods of grom;h. 12- The optimum duration of light period for maximum flowering was 11.22 hrs/day. Growth substances and micronutrients seemed to reduce number of opened flower/plant. 13- GA 3 100 ppm with Fe,Zn or ~mincreased· different y.ield components, while with eu or (Fe + Zn + Mn + eu) decreased such components. 14- The application of NAA + most micronutrients decreased yield componellts of roselle plant. - ---- -- _._-~~~--~~~~~~~~~---- - -_.--_.---- - - - - _. -- -- -_. - - -_._---- -104- 15- NAA + GA3 at 100 ppm decreased different yield components of roselle except fiber yield. 16- The addition of Fe or Zn beside GA3 and NAA increased different yield components of roselle. 17- The highest stem yield of roselle was produced by the application of GA3 100 ppm or NAA 25 ppm or micronutrients. 18- Sepals yield of roselle was increased by applying micronutrients alone or combined with GA3 50 ppm. 19- Seed yield of roselle increased significantly by GA3 at 50 ppm or 100 ppm + micronutrients. 20- Increasing NAA concentrations up to 50 ppm with or without micronutrients decreased roselle seed yield. 21- Growth substances (GA3 & NAA) at both rates increased fiber yield of roselle significantly. Such increase was stimulated by the addition of micronutrients. 22- Oil percent in roselle seeds was not affected by both growth substances and micronutrients. 23- The application of micronutrients·stimulated the the accumulation of total anthocyanin in sepals of flowers ~nd flowerbuds but slightly decreased it in mature fruits. 24- GA3 application at both rates (50 & 100 ppm) accelerated the anthocyanin formation in different reproduc- -- -- --- ------- ---~ -105- tive organs. 25- The relative proportion of residual non-soluble protein was the highest protein fraction in roselle seeds. 26- The application of growth substances (GA3 & NAA) and micronutrients increased the proportion of residual non-soluble protein, while decreased both water soluble and ethanol soluble protein. 27- The following amino acids were identified in the different protein fractions of roselle seeds: valine,8.1anine,isoleucine,glYCine,leuCine,serine,threo_ nine,aspartic aCid,glutamic,acid,lysine,arginine,histi_ dine,cystine,methionine,proline,tyrOsine and phenylalanine. 28- Proteinogenic amino acid concentrations varied greatly with the different protein fractions. 29- Tyrosecine and cystine were absent from most protein fractions and when present were in trace amounts. 30- Proline was found to be the dominant amino acid in most protein fractions. 31- Proline amounts increased by the application of NAA while GA3 decreased its concentrations • 32- GA3 and NAA enhanced the accumulation of alanine CLnd methionine in different protein fractions. ----._--- --” -- .- ._-._._------_ .. -- -- ------- -106- 33- The combination of micronutrients and ~A3 or NAA increased proline amounts in different protein fractions. While such application decreased alanine and methionine concerrt.ret Lon, |