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Abstract
The present study aimed to: study the effect of different subchronic doses of
gamma irrradiation on protein and isozymes of the larval (1st, 2nd and 3rd instars) and pupal stages of Musca domestic, and investigate the mtDNA pattern of pupal stage after gamma irradiation with different subchronic doses.
I. Protein electrophoresis of irradiated housefly larvae and pupae
1.1. Protein electrophoresis of irradiated housefly 1st larval instar
Protein fractionation of unirradiated 1st larval instar produced 3 bands. When
these larvae are exposed to different doses of irradiation (2.5, 5, 10, 20, 30 GY), the three bands were disappeared in samples exposed to the high doses of
irradiation (20, 30 GY). On the other hand, 15 unique bands were appeared when
larval samples were exposed to the different doses of radiation.
The similarity index between the control and the irradiated samples (0.0-0.22), and between the irradiated samples themselves (0.0- 0.5) recorded low values, indicating the apparent effect of irradiation on irradiated samples and the visible difference among the used doses.
1.2. Protein electrophoresis of irradiated housefly 2nd larval instar
Protein fractionation of unirradiated 2nd larval instar produced 2 bands.
Interestingly, these two bands disappeared completely when larvae exposed to the different doses of irradiation (5, 10, 20, 30 GY). In contrast, 18 unique bands were appeared when larvae exposed to the different doses of irradiation.
The similarity indices between the control and the irradiated samples and
between the irradiated samples themselves, recorded low values (0.0- 0.35) indicating the apparent effect of irradiation on samples and the apparent effect of the different irradiation doses among themselves.