الفهرس | Only 14 pages are availabe for public view |
Abstract Although the histology, ultrastructure, histochemistry and immunohistochemistry of the spleens of different species were studied long time ago, the spleen of the one humped camel was only shortly described histologically and histochemically This work aimed to study the histology, ultrastructure, histochemistry and immunohistochemistry of the camel spleen and to describe its compartments and their cellular composition The study was carried out on 68 spleens obtained from clinically healthy camels (Camelus dromedarius) of both sexes (6 months to 15 yeas old). The specimens were used for the following studies: 1. Routine histology: Using H&E, Crossmon trichrome, Orcein, Gomori’s silver impregnation, PAS and Giemsa stains. 2. Ultrastructure: Specimens from 15 camel spleens were studied using both of the TEM and SEM. 3. Histochemistry: The acid and alkaline phosphatase enzymes were studied in specimens from 15 camel spleens. 4. Immunohistochemistry: Antibodies specific for bovine (n = 193), swine (n 14) and human (n = 6) leukocyte differentiation antigens were tested immunohistochemically for cross-reaction with camel cells in 15 spleens using immunofluorescent and PAP techniques. The cross reacted antibodies were used to study the immunoarchitecture of the camel spleen and the topography of the lymphocytes and platelets in its compartments. The histological study showed that the camel spleen was surrounded by a highly muscular thick capsule divided into a thinner outer layer (about 1/3 of the capsule thickness). |